Sentence examples for vector and used from inspiring English sources

These sequences were separately cloned into the pEXP5-CT/TOPO expression vector and used for transfection of BL21 DE3) cells.

In August 1983, the two researchers reported that they had built the vector and used it successfully to introduce a functioning gene for human HPRT into rodent cells in vitro.

subtilis shuttle vector and used to clone and express protein.

Close to one million bp of nuclear DNA sequences from one bone were directly determined by high-throughput sequencing on the 454 platform (40), whereas DNA fragments from another extract from the same bone were cloned in a plasmid vector and used to sequence ~65,000 bp (42).

These fragments were cloned into the Green Pelican vector and used to create transgenic fly strains via standard techniques.

All sequences were then cloned into the pCAGGS expression vector and used for expression testing and immunization [10].

Amplified DNAs were re-cloned into the lentiviral vector and used to make fresh viral stocks containing the enriched shRNA sequences.

We first checked whether NUMTs enhanced the replication activity of an ectopic vector, and used pIRT2Δ, a Schizosaccharomyces pombe vector that replicates very poorly in S. cerevisae.

Transposons were released from the vector and used to prepare transposomes for electroporation of rickettsiae according to the manufacturer's suggested protocol.

The library is based in a retroviral vector and uses convergent promoters to produce unique small complementary RNAs.

These properties are then arranged as feature vectors and used as input to classification techniques [7], [19] [24].