Sentence examples for vector and tested from inspiring English sources

HEK293T cells were transfected with appropriate expression vectors carrying the human CHKA or CHKB genes or an empty vector, and tested for ChoK and EtnK activity.

Different ULS1 fragments were constructed into a Gal4-AD yeast two-hybrid vector and tested for interaction with BD-Slx5.

We transduced MSCs with the IL-28A gene using a replication-incompetent AdF35 vector and tested whether the transduced MSCs produced cytotoxicty to tumor cells co-cultured.

Three new shRNAs (S1, S2 and S3) were designed to target the coding sequence (CDS) of CYP3A4, cloned into a shRNA expression vector, and tested in different cells.

Plasmids were isolated, retransformed into BTH101 carrying either pKT25_ dynA or the empty vector and tested on BTH medium to sort out false positives.

The ≈1,000-bp ≈1,000-bp0-bp PCR products from strand 00LA1 were cloned independently into the pCR 2.1 vector and tested to determine if recombinant plasmids confer trimethoprim resistance after transformation to Escherichia coli.

We detail how to clone candidate genes into this vector and test its functionality in Drosophila by targeting a gene coding for the fluorescent protein DsRed.

We therefore re-engineered the TFIIE subunit production vectors and tested various Escherichia coli host strains to optimize expression.

The following strains were transformed with the different vectors and tested for expression: E. coli BL21 DE3), E. coli BL21 DE3 pLysS, E. coli Rosetta DE3), E. coli Tuner DE3) and E.coli Tuner DE3 pLysS (Novagen).

To study the effect of the microdeletions on IC1 function, we amplified the mutant IC1 alleles from patient DNAs, cloned them into plasmid vectors and tested their functional properties by transferring them into cultured cells.

Strain Isoy16 was transformed with plasmid p425-synthILV235 and as negative control with an empty vector, respectively, and tested for valine and isoleucine prototroph.