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Transformation into a bacterial vector and subsequent protein expression was performed by Dr. Cynthia Kinsland.
We confirmed successful integration of this PE targeting vector and subsequent removal of the selection cassette.
Apoptosis induced by this vector and subsequent uptake of apoptotic cells by DCs probably also contribute to the potent response.
In order to exclude such random effects, permutation testing consisting of 1000 independent random shuffles of the label vector and subsequent determination of BACC was performed.
Transformation into a bacterial vector and subsequent protein expression was performed by Dr. Cynthia Kinsland and the Cornell University Life Sciences Core Laboratories Center Protein Production Facility.
First we transfected pools of barcoded vectors into three parasite clones, each of which already carried a different barcode from the insertion of a targeting vector and subsequent recycling of the selection marker.
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However, constructing retrotransposon assay vectors and subsequent mapping of integration sites remain technically challenging aspects of the field.
In vitro release of rAAV vectors and subsequent transduction of human mesenchymal stem cells (hMSCs) by rAAV vectors from the release medium and from gels in direct contact with the cells were investigated.
Double knockdown cells were obtained by sequentially infecting two shRNA expression vectors and subsequent selection using 1 mg/ml G418 and 10 μg/ml blasticidin (VWR).
At this age, the animals are usually released onto the pasture for grazing, where they are likely to be exposed to infected vectors and subsequent BTV infection.
The promoter sequences of five genes (HRAS, NTRK2, PDGFA, IGF1R, and SRC) with >15% of output to input, and the promoter sequences of p53 as a negative control were selected for cloning into expression vectors and subsequent analysis.
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