Sentence examples for vector and shRNA from inspiring English sources

The PCR products were cloned into pEGFP-N1 vector and shRNA expression vectors were constructed.

Hairpin pLKO.1 control vector and shRNA expressing plasmids for knocking down PKAcα expression plasmids (PKAcα kd #1 and #2) were obtained from Open Biosystems.

Adding the requested ChIP-seq data now would require performing three replicates of ChIP-seq for all factors not only in the empty vector and shRNA-induced conditions, but also in the lines expressing SCML2 WT and ΔRBR, because otherwise quantitative comparisons between different experiments would be difficult to interpret.

High-quality, low-endotoxin plasmid preparations (QIAGEN) of GFP expression vector (pCIG2) and shRNA vectors were injected at 1 μg/μl for each plasmid, together with Fast Green (0.05%, Sigma).

MGC-803 cells were transfected with LEIGC vector or LEIGC shRNA vector and scrambled shRNA or non-related lncRNA vector, and incubated for 24 h.

We used H460 cells containing empty shRNA vector and NPC2-shRNA.

Immediately after ischemia was induced, either a total of 40 µg Sproutys shRNA vectors (20 µg Sprouty2 shRNA vector and 20 µg Sprouty4 shRNA vector) or a quantity of control shRNA vectors was injected into five different sites in the adductor muscle of each anesthetized mouse [32].

For mouse p53 knockdown, the p53 short hairpin RNA (shRNA) vector and its human Rb shRNA control vector were kindly provided by Dr. SW. Lowe (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA).

Both the overexpression vectors and the shRNA vectors encode blasticidin resistance cassettes, so the selection and maintenance are the same for both sets of lines.

Plasmid vectors and Anxa6 shRNA constructs were acquired from Clontech and OriGene Technologies (Rockville, MD, USA), respectively.

For JMJD2B shRNA, the pLKO.1 puro (Addgene plasmid #8453) [27] lentiviral vector and control scrambled shRNA (Addgene plasmid #1864) [28] were used.