Sentence examples for vector and maintained from inspiring English sources

Hep3B cells were stably transfected with full length Pyk2 or PCDNA 3.1 empty vector and maintained in DMEM medium supplemented with 300 µg/ml G418 as described previously [15].

Transfection with mCherry was done using mCherry lentiviral vectors and maintained in DMEM (Lonza) with 10% fetal bovine serum (FBS) and 1% Pen/Strep.

Interestingly, the piPSCs contained abundant episomal vectors and maintained high-level transgene expression even after multiple passages in the N2B27 medium supplemented with small molecules (Fig. 2C and Fig. S2B), suggesting a likely mechanism that these small molecules improved episomal reprogramming was through the retention of episomal vectors and transgene expression.

Treatment was initiated prior to vector exposure and maintained during subsequent co-culture with 293T cells.

For atherosclerosis regression studies, three groups of male mice (n = 10 per group) were given unrestricted access to water and were fed a high-fat western diet (0.15% cholesterol, 21% butterfat; DYETS, PA, USA) starting 8 weeks prior to vector injection and maintained on this diet throughout the experiment.

About 2 × 105 HepG2 cells were transiently transfected with 0.3 µg hNTCP/pcDNA6 or a vector control and maintained in PMM.

Cells with the ura4-D18 mutation were transformed with the linear vector DNA and maintained on minimum medium without uracil to select for Ura+ transformants.

Stable transfected lines were generated by transfecting no insert (pcDNA3) and pdDNA3-API5 vectorselectedted and maintained in the presence of appropriate concentrations of Zeocin™ (Invitrogen).

This strategy has several potential advantages over conventional gene therapy including eliminating the need for immunosuppression, avoiding the risk of insertional mutagenesis by therapeutic vectors, and maintaining expression of the corrected gene by endogenous control elements rather than a constitutive promoter.

Retroviral pseudotyping for gene transfer applications endeavors to alter vector tropism and maintain a suitable titer.

These viruses are not subject to stem cell-specific viral silencing due to the advanced vector design and maintain the expression of the transduced genes after neuronal differentiation.