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Both vector and low quality sequences were trimmed off the original sequences.
The electropherogram sequencing files were processed using the Phred program [46] for base calling and for trimming of vector and low quality (<20) sequences.
Unknown vector and low complexity masking was performed by SeqClean.
After trimming vector and low quality sequences the average PHRED 20 read length was 693 bp.
Following removal of vector and low quality sequences, all sequences ≤ 50 bp in length were discarded.
Base calling was performed using TraceTuner and sequences were trimmed for vector and low quality sequences using Lucy [ 25].
Similar(44)
The electropherogram files generated by sequencing were processed using the Phred program [31] for base calling and trimming of vector and low-quality (<20) sequences.
In this process adaptor, vector and low-quality regions are removed.
Sequences were trimmed for vector and low-quality sequences with Seqtrim V0.110 [ 37].
The 454 sequences for each species were trimmed of vector and low-quality sequences, and then clustered by Agencourt.
Recombinant clones were sequenced using Big Dye Terminator chemistry and ABI 3730xl sequencers (Applied Biosystems, CA), and vector and low-quality sequences were electronically trimmed.
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