Sentence examples for vector and inserts from inspiring English sources

Vector and inserts were purified using the Qiagen MinElute Kit or Gel Purification Kit following the instructions of the manufacturer.

Next, the PCR products served as mega-primers for simultaneous assembly of the vector and inserts by linear amplification.

Ura et al. (2009) adopted adenovirus Ad5 as the vector and inserted 2F5 epitope sequence in the HVRS region of envelope protein.

Vector and insert were gel purified using QIAEX II (QIAGEN, Valencia, CA) and ligated using T4 DNA ligase (Roche).

The vector and insert were mixed at 1∶3 molar concentrations and ligated at 16°C for 16 h.

The PEG10 5'-RACE PCR products were subsequently cloned into a vector and insert sequences were analysed by sequencing.

The K299R PAK1 insert was removed from the pCMV6M vector and inserted into the multiple cloning site of the pEGFP-N1 vector.

The digested vector and insert were ligated and transformed into E. coli DH5α competent cells and plated onto LA-carbencillin plates.

Before co-transformation both vector and insert were linearized by PCR.

The main limitation seems to be the vector and insert length.

It is based on the introduction of overlaps between the vector and insert by PCR.