Sentence examples for vector and heavy from inspiring English sources

For proteomics analysis, whole cell extracts isolated separately from "light" (empty vector) and "heavy" (eIF6 over-expression) cell lines were mixed in equal amounts.

In order to circumvent these problems, single domain heavy chain cDNAs were subcloned into a mammalian expression vector and heavy chain antibodies (HCAbs) made up of variable heavy chain regions fused to mouse Fc region were generated.

Here we have developed a dual lentiviral vector strategy for the genetic engineering of cell lines compatible with macroencapsulation, using separate vectors encoding IgG light and heavy chains.

Primers annealing to pComb3X vector or heavy and light chain constant regions (Table S1 and S2) were used to amplify the scFvs from the gDNA antibody libraries and Fds and light chains from the cDNA antibody libraries.

This design of a single cassette vector combining heavy and light chain expression elements allowed rapid production and secretion of correctly processed and assembled intact immunoglobulins from recombinant baculovirus infected insect cells.

Vectors encoding the light and heavy chains of a proprietary human IgG1 antibody were kindly provided by Dr. Burkhard Wilms at Novartis.

Here we examine an antibody-expressing Chinese hamster ovary (CHO) clone at single-cell resolution using flow cytometry and vectors, which couple light and heavy chain transcription to fluorescent markers.

Each immunogen was produced in Nicotiana benthamiana plants using twin magnICON vectors expressing the light and heavy chains of the idiotypic Ab.

Synthetic DNA fragments and human antibody constant region cDNA were used to construct two CMV promotor-based expression vectors for the antibody light and heavy chains, in which the variable region was connected directly to the constant region without an intron sequence.

Expression vectors containing ch-mAb6B5 light chain and heavy chain cDNA were constructed and expressed in the murine myeloma cell line P3X63-Ag8.653.

The light and heavy chain genes were subcloned in vector gWIZ (Genlantis), engineered for protein expression by transient transfection of mammalian cells.