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Importantly, we observed lower expression of AVEN when overexpressing miR30a (pre-miR30a) in both conditions, empty vector and full length AVEN transfected cells.
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Amplicons were cloned into a sequencing vector, and full-length ORF sequences were obtained.
Full length and truncated MID1 cDNA were cloned into the pSOS vector and full-length EF-1α cDNA into the pMYR vector.
Then, we transiently co-transfected HNSCC cells with the CerS1 or CerS6 promoter-luciferase reporter vectors, and full-length beta-galactosidase (β-gal) expression vector (used to normalize transfection efficiencies) to measure their activities.
The state-of-the-art modified quadratic discriminant function (MQDF) based approach for online handwritten Chinese character recognition (HCCR) assumes that the feature vectors of each character class can be modeled by a Gaussian distribution with a mean vector and a full covariance matrix.
For each facet, the surface normal, the scattering vector, the point at which the ray touches the ground, the backscattering vector, and the full range path are computed as follows.
Full-length cDNA of G9a, GLP, and WIZ was cloned into pS-FLAG-SBP (SFB) vector, respectively, the full-length cDNA of ZNF644, G9a, and GLP was cloned into pCMV-Myc vector, and the full-length cDNA of WIZ was also cloned into pCMV-HA vector.
The pIRES2-ZsGreen1 vector was used to construct a pIRES2-ZsGreen1-TAp63 pIRES2-ZsGreen1-TAp63 pIRES2-ZsGreen1-TAp6363 α were used as the template.
To identify the interaction domain of ZPAC with Ump1, partial ZPAC cDNA fragments (aa (amino acids) of 1 88, aa 89 176, aa 174 264, aa 265 351 and aa 1 351) were PCR-amplified and subcloned into the p Gilda LexA vector and the full-length ORF sequence of mouse Ump1 was PCR-amplified and subcloned into the p B42AD vector.
We assume that the relay nodes perfectly know the channel state information (CSI) of H i,j. The relay node R i performs the optimizations of F i and V i and then transmits the information to the source nodes 1 and 2. Since source node i knows its own transmitted signal vector s i and full CSI, the self-interference components in (3) can be efficiently canceled.
TSAd cDNA was cloned into the pEF-HA expression vector, and constructs encoding full-length TSAd [ 39], TSAd ∆239-274, TSAd ∆Exon 7 (aa 239 334) [ 18], single, double and triple TSAd tyrosine (Y) to phenylalanine (F) mutants [ 5] were cloned as previously described.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com