Sentence examples for vector and determined from inspiring English sources

Therefore, we transiently transfected differentiated SH-SY5Y cells with an hClC-6 expression vector and determined the distribution of overexpressed hClC-6 by means of CLSM.

We constructed the mouse MIP-1β promoter (-1,989 to +68) into pGL3-basic vector and determined whether AR can activate the MIP-1β promoter.

To determine whether these heterodimers could activate a PPAR-responsive promoter, we transiently transfected four breast cancer cell lines with a heterologous PPAR promoter vector and determined reporter gene activity in response to selective ligands.

In the same experiment, we also assayed the biological activity of the unconstrained 12.1 and Arf37 peptides expressed from the retroviral vector and determined if the addition of a nuclear targeting signal enhanced the activity of the CI2-presented peptides.

Therefore, we transduced primary mouse B cells as well as primary leukemic cells derived from TCL1 mice with an AID-SRC-encoding murine retrovirus vector and determined AID splice variants by performing RT-PCR using PCR primers specific for endogenous murine AID and AID-SRC (Supporting Information Tables 2 and 3), respectively.

In order to further study the role of MCL-1 in the induction of cell death by sorafenib, we have transfected PC3 cells with the MCL-1 expression vector and determined caspase 3/7 activity after treatment with sorafenib (Supplementary Figure 2, see section on supplementary data given at the end of this article).

To utilize the PSO+ algorithm, we need to represent a solution as a vector, and determine a fitness function appropriate for the problem.

We then use Bayesian model averaging to combine the evidence from each pseudo-data vector and determine the probability of DE.

The inserts were sequenced with vector primers and determined to be consistent with FlyBase transcript annotations "RA" (1.2 kb form) and "RB" through "RF" (1.8 kb form).

To address this, we transfected PC3 cells with a non-degradable DN mutant of I κB- α (Brown et al, 1995) (DN-I κB- α) or a control vector (pCMVNeo) and determined whether this could modulate the effects of IP6.

To achieve the optimal duty cycles, an ATPG-like algorithm is proposed to generate multiple input vectors and determine the applying time for each vector.