Sentence examples for vector and cells from inspiring English sources

For co-precipitation experiments, 293T cells were transfected with a total of 20 µg of plasmid DNA (10 µg of each construct or empty vector) and cells were harvested 48 h later.

To test this prediction, Vero cells were pre-loaded with 0 to 250 pfu per cell of Ad-ICP4 or a null control vector, and cells were inoculated 12 hours later with HSV-1 0−GFP in the presence of cycloheximide.

The [Ca2+]i of UAMS-32 cells with stable expression of either HA-Arl6ip5 or control vector and cells transiently transfected with Arl6ip5-siRNA (Thermo Fisher Scientific Inc).

Planktonic Growth Study: The isometric growth of the isolates were measured by constructing growth curves of the negative control (i.e., empty display vector) and cells displaying peptides Al-S1, DABD1, DBAD24, DBAD8, and DBAD14 in either LB+Cm, LB+Cm with an arabinose induction described previously, or in LB+Cm/Glu.

Cells injected into the #4 (left side) mammary fat pad of mice were labeled with dual-luciferase-Tomato red reporter gene (pFULT) vector and cells injected into the #9 (right side) mammary fat pad of mice were labeled with dual luciferase-GFP reporter gene (pFULG) vector.

To determine whether changes in oxygen tension regulate HIF-α at the protein level, HEK 293 T cells were transfected with either the indicated Myc-tagged HIF-α constructs or an empty vector, and cells were subjected to 18 h of hypoxic treatment after 6 h of normoxic cultivation.

The cells can be represented in a one-dimensional vector, and cell C i,j can be represented by c t and the value of t can be computed in the following way.

To this end, cells were transfected with C-terminally V5-tagged cullins or empty vector and cell lysates subjected to V5 immunoprecipitation.

Each vector and cell line combination was tested in 3 replicate experiments.

Among them are molecular biologists and protein engineers who make the receptors, immunologists who do the in vitro and preclinical research, virologists who make the viral vectors, and skilled specialists who can manufacture vectors and cells according to Good Manufacturing Practice guidelines.

Human PM1 cells, which are permissive to HIV infection [ 41], were then infected with these vectors, and cells expressing the EGSs were cloned.