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Kang and Fu also established a functional differential equation SIS model with an infective vector and analyzed the global stability of the endemic equilibrium, the disease-free equilibrium [22], and so on.
The PCR products were cloned into a pGEM-T vector and analyzed by direct sequencing.
Stained sections were mounted (Vector) and analyzed on a Leica confocal microscope.
Each MEKK1 mutant was overexpressed in 293T cells in the highly expressing pApuro vector and analyzed via western blot to check for protein expression.
To confirm that the PCR product indeed represents human troponinT, we sub-cloned the human-troponin T PCR products using pGEM-Teasy vector and analyzed the sequences.
Mutants were transiently expressed using the pSG5 expression vector and analyzed by Northern blotting.
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Microsatellites in human TGFBR2, PTEN and APC genes were placed in yeast vectors and analyzed in isogenic yeast strains that were wild-type or deletion mutants for MSH2 or MLH1.
In order to down-regulate A3G, we transduced activated CD4+ T cells with shRNA/Vif-expressing vectors and analyzed their susceptibility to HIV1 infection once they had returned to a quiescent state.
Cho W-K and Yoo J constructed the adenoviral vectors and analyzed the functionality of the expressed gene products.
SW620 CRC cells were stably transduced with miR-143 or miR-145 expression vectors and analyzed in vitro for cell proliferation, cell differentiation and anchorage-independent growth.
We generated stably transfected PC12 cell lines with short-hairpin RNA (shRNA -expressing vectorshRNA -expressinge capacity of each RNAi to knock down endogenous Ack1 expression in PC12 cells.
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