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The issue raised, then, is: Are both measures contaminated by the length of the vector analyzed?
Cells were covered with mounting solution (Vector), analyzed field by field and examined using an Olympus IX2-UCB microscope using 20x and 40x objectives.
ZD constructed the transgene expression vector, analyzed the results and reviewed the manuscript.
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Moreover, the vector analyzing powers of the PDRC reaction are rather sensitive to MECs, making them a good testing ground for the details of exchange currents.
Among the three different vectors analyzed in this study (i.e., pET28a, pET22b, and pET32a), pET28a, carrying the lacB gene, showed the best results regarding expression levels, cell growth, and enzyme activity at different pHs.
This difference may reflect the kinds of gene expression profiles (vectors) analyzed here.
RC drafted the manuscript, conducted molecular cloning, carried out functional and phylogenetic analyses of EuIPI, constructed vectors, analyzed overexpression of EuIPI in E. ulmoides, regenerated transgenic plants, and conducted DNA/RNA analyses.
39 The R-factors quoted are defined as R=(∫[ χT− χE] kndk/[ χE] kndk)⋅100%%, in which χT and χE are the theoretical and experimental EXAFS, k is the photoelectron wave vector, dk is the range of photoelectron wave vectors analyzed, and n is the weighting in k that was applied to the data.
The cell lysates prepared from the cells transfected with empty vector (mock) and NvHsTRPA expression vector were analyzed by Western blot using rabbit anti-V5 antibody and HRP-conjugated anti-rabbit IgG antibody (GE healthcare, 3000 fold dilution).
Preliminary differential cross sections and vector analyzing powers are presented and results are compared with state-of-the-art three-nucleon calculations.
In the present work, we explored 3NF effects in the break-up scattering process by performing high precision measurement of vector analyzing powers and differential cross sections using a 190 MeV polarized-proton beam on a liquid deuterium target.
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