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Solutions of DNA containing one each of the seven CCV ORFs, vector alone or PBS were injected intramuscularly into 4 8 cm catfish.
CV-1 cells were cotransfected with a GRE-SEAP, and GR500 expression vector alone or with added SRC-1.
To investigate the effects of H5N1 NS1 expression on IFN signaling, HeLa cells were transfected with either vector alone or plasmid containing HA-tagged H5N1 NS1.
No metabolite of alprazolam was detected in cells transfected with vector alone or vector containing the cDNA in reverse orientation (data not shown).
To test this predication, ctf7 single mutant and ctf7 elg1 smc3Q triple mutant cells were transformed either with vector alone or vector providing for elevated PNCA expression.
Single clones containing either the vector alone or pHybrid-5FU55 were obtained following selection and characterised for resistance to 5-FU using a clonogenic assay.
HeLa cells stably expressing wt-CFTR were transiently transfected with Myc-COMMD1, vector alone or COMMD1 siRNA and subsequently incubated with the protein synthesis inhibitor cycloheximide for different time intervals.
Fig 5 shows that on selective media we detected a strong interaction between TTC4 and HuCDC6, while no interaction was seen when TTC4 was co-transfected with the vector alone or the human DNA polymerase alpha.
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For this purpose, MCF7 cells were infected with Ad5hTRAIL or AdIKKβKA vectors alone or in combination.
This BSXEIE construct was cotransfected with Brn-3b or ERα expression vectors, alone or together, into MCF-7 cells.
Thus, we selected SGC7901 as the test cell line and transfected the cells with the MAWD and MAWBP eukaryotic expression vectors that we constructed; the cells were transfected with each of the vectors alone or with both vectors.
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