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Akt-knockout MEFs were infected with adenovirus expressing Akt and adenovirus vector alone as control.
In these experiments, we define the expression level of the vector alone as 100%.
PUF60rm cell lines were generated by retroviral transduction with pMarx-PUF60rm or vector alone as described [65].
To do this, HEK293 cells were transfected with UBE3A expression plasmids or the empty vector alone as control.
HEK-293 cells grown on coverslips were transfected with recombinant constructs expressing mouse NCKX4 or vector alone as a negative control.
As shown in Fig. 3B, TGFβ3 blocked PDGF-bb-stimulated migration of HDFs infected with vector alone, as expected (bar 3 versus bar 2).
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We set MI-GRAAL to use graphlet degree vector similarity (GDS) alone, as well as to use GDS coupled with sequence similarity (GDS+SEQ); since it is a randomized algorithm, we repeat each alignment process 15 times for GDS and 15 times for GDS+SEQ, to find alignments of the best topological and biological quality.
By comparison with the activity of the pGL3 vector alone normalized as 1, the human, mouse, and canine genomic fragments increased the luciferase activity by approximately 50-, 300-, and 100-fold, respectively, in the CDT2 direction (Fig. 2B).
Cells transduced with empty vector alone served as controls.
The GFP vector alone served as a control.
In all experiments the level of cell spreading detected in cell expressing vector alone served as a baseline to cell spreading in EPAC1-expressing cells.
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