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where α i is the positive Lagrange multiplier, x i is the support vector (a total of N) and K(x i, x) is the kernel decision function.
For each transformation vector, a total of 10 primary T0 transformants from five independent transgenic lines were first screened for the presence of CX3 or CX5 transgene by PCR.
From each vector, a total of 30 clones on a selective plate with karnamycin were picked one-by-one; every clone was transferred into 1.0-mL selective medium in a 5.0-ml Ependorf tube for cultivation at 37°C for 3 h.
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We consider two settings each of sample size and mixing proportion vectors (a total of four settings).
For the null distribution simulations, we consider two settings each of sample size and mixing proportion vectors (a total of four settings).
We also included E. coli carrying the empty pL4440 vector, for a total of 43 RNAi vectors in the survey.
The length of the pCR®2.1-TOPO® vector was 3931 bp; thus, the cloned vector was a total of 4419 bp in length.
HEK293 cells were and transfected using Dreamfect (OZ Biosciences), 10 ng of luciferase vector and a total of 200 ng of protein expression vector were used per 96 well.
For the overexpression studies, each condition consisted of 25 ng CMV-Luc internal control, 25 ng of each of the GABP expression vectors or their corresponding empty vector controls, and 175 ng of the specified renilla luciferase reporter vector, for a total of 250 ng of DNA per well.
The pEPI-1 vector contains a total of 305 CpG motives, most of them within the vector elements necessary for bacterial propagation.
Consequently, the per frame feature vector comprises a total of 94 features.
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