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BAT for mature adipocyte and stromal-vascular fractionation was excised from 6-week-old female mice into DMEM before mincing with sterile surgical scissors and digestion at 37°C with shaking for ∼45 min (10 ml Hanks buffered salt solution, 2% BSA, 2 mg/ml Type 2 collagenase).

In keeping, epididymal adipose tissue fractionation experiments showed that Cnr2 was predominantly expressed in the stromal vascular fraction, both in lean ob+/ob and obese ob/ob animals, and was barely detectable in the adipocyte fraction (Fig 1B).

Tissue fractionation experiments show that enhancement of Cnr2 expression arises from the macrophage-enriched stromal vascular fraction of the adipose tissue and the non parenchymal liver cell fraction, whereas Cnr2 expression in adipocytes and hepatocytes remains negligible.

stromal vascular fraction.

Fractionation of whole adipose tissue into adipocyte fraction (AF) and stromal-vascular fraction (SVF) was via collagenase digestion and differential centrifugation, as previously described [ 26- 28], starting with pooled tissue of 6 mice.

20 fractions were obtained after fractionation.

Multiple rounds of WGD events (both allopolyploidy and autopolyploidy) cyclically increase the genetic diversity in vascular plants, and subsequently this is steadily eroded by genomic fractionation toward diploid-like genomes.

Fractionation by anion exchange gave fractions with lower activity compared to cation exchange fractionation (Table  S7).

Fractionation also occurs in enzymatic processes like photosynthesis.

This technique is called field-flow fractionation.

Many natural processes discriminate among these isotopes, a phenomenon called fractionation.