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stromal vascular fraction.
Stromal vascular fraction cells were isolated following established protocols [14].
Here, we evaluate autologous adipose-derived stromal vascular fraction, called SVFs.
Stromal vascular fraction (SVF) represents an attractive source of adult stem cells and progenitors, holding great promise for numerous cell therapy approaches.
A20 Using naturally occurring large animal models of human disease to enable clinical translation: treatment of arthritis using autologous stromal vascular fraction in dogs.
Accordingly, a mean of 4.8 × 107 stromal vascular fraction cells, which contained a mean of 4.0 × 106 stem cells, were used for MSC injection.
[18F]FLT SUV has been shown to be highly correlated to vascular fraction and perfusion/permeability soon after tracer injection [16].
Thus, after isolation, the adipose-derived stem cells represented a mean of 8.7%% of the stromal vascular fraction cells (range, 6.9 to 11.2 %).
To evaluate the frequency of mesenchymal-like progenitors in the stromal vascular fraction, cells were cultured in T-25 flasks at a final concentration of 16 cells/cm2.
Adipose tissue is composed of adipocytes and the stromal vascular fraction containing adipose tissue macrophages (ATMs), adipose stem cells (ASCs) and Muse-AT cells, among other cell components.
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Contaminating erythrocytes were eliminated from the stromal-vascular fraction by a wash with sterile distilled water.
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