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Others have also investigated the effect on the mouse kidney with varying experimental protocols and results [8, 11].
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These associations were consistent across two years, three lineages, and varying experimental design protocols.
The varying experimental setups may produce different results.
These observations are useful indicators of protocol validity; though, the ultimate fidelity criterion is the ability to maintain differential expression between different tissues or under varying experimental conditions.
Three experimental protocols were designed varying the inoculation sequence.
The scheme of the experiment is in analogy to the study of Richter et al. [7]; 8 × 8 animals underwent 18F-FDG scans according to PET protocol 1. Varying experimental parameters were gender, age, and cage occupancy of the animals (n = 2 or n = 13 per cage).
Not only are the deep sequence DNase-seq datasets available at varying sequence depth, they are also generated by two different experimental protocols: a single-hit version [ 8] and a double-hit version [ 25] of DNase-seq.
However, in an experimental setting, the test may not be ideal due to the varying effects of the muscle venous pump among different participants in some experimental protocols and the high rate of false positives in adolescents (De Jong-De Vos van Steenwijk et al. 1995).
The Deakin University Animal Ethics Committee approved all experimental protocols.
Several experimental protocols are described and implemented.
Experimental protocols were approved by the Norwegian Animal Research Authority.
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