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Whereas changes to the chromophore moiety exhibited slight to moderate changes to the hydrophobic nature of these molecules, substitution at the alkyl indolium side chain has enabled us to vary the binding affinity towards serum albumin.
The substrate-binding module can vary in three ways: typical kinases that preserve fold and merely vary the residues in the substrate-binding groove, typical kinases than rely on other regions/regulatory proteins in addition to the substrate-binding groove, and atypical kinases that vary the binding mechanism all together.
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The binding energy of the relevant core electrons is element specific, but differences in chemical bonding vary in the binding energy value by +/− 0.1 eV to a few eV.
Although the lengths of the flexible β-hairpin arms vary, the central presumed DNA-binding surfaces are all distributed with positively charged residues predominantly.
As the type of amino acid was varied, both the binding strength between amino acid and GO and the moduli (G′ and G′′) of reaction mixtures followed the same order, arginine > cysteine > tryptophan > asparagine > aspartic acid > glycine.
While the identity of the metal varies, the binding site is well-conserved at the superfamily level with an aspartic acid and two histidine residues acting as ligands to the metal.
Changing the concentration of heparin varies the number of available binding sites to the incorporated growth factors and as a result the release rate of growth factors can be modulated by consequently increasing its retention time within the fibrin matrix [ 49].
Notably, the source of erythrocytes to detect influenza virus may vary, depending on the binding specificity of the hemagglutinin protein for each virus.
To probe the requirements for self-association and heparin binding, we varied the chain length and the nature of the N-and C-terminal functional groups (compounds 2– 6; Figures 1B, C).
In the case of oligonucleotide arrays, the individual oligos representing a gene vary in binding affnity (i.e. sensitivity) and specificity and the annotation of individual spots/probe sets varies in reliability.
The ADLs differ from programming languages in the sense that the latter bind all architectural abstractions to specific point solutions, whereas ADLs intentionally suppress or vary such binding.
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