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We compared the expression level of OsFC1 in tiller bud in the various transgenic lines and found that OsFC1 was down-regulated in OX lines and was up-regulated in Ri lines and mutant (Fig. 6).
To investigate the possible interaction between nitrogen and plant hormones, we measured the expression levels of the important genes responding to CK and SL pathways in the rice tiller bud with various transgenic lines.
After the transformation, more than 10 transgenic lines displayed early flowering and stunted growth, and this early flowering phenotype associated with OsMADS45 expression observed in the progenies of various transgenic lines was analyzed and verified for more than three generations.
Statistical significance of the spot count, spot area and spot intensity values between N2 (wild-type) and various transgenic lines in Figure 2 and dose-response in Figure 6 was determined using an unpaired, one-tailed, Student's t-test.
Seeds from Arabidopsis thaliana Columbia (Col-0) ecotype or various transgenic lines were sown on Murashige and Skoog medium, stratified at 4°C for 3 d and incubated at 22°C under continuous light.
All vectors except the most recent NLS derivatives have been used to generate various transgenic lines.
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We successfully produced various transgenic rice lines showing enhanced tolerance to low Fe availability by introducing barley MAs biosynthesis genes.
Total DNA from various independent transgenic lines was extracted (Dellaporta et al. 1983) and screened by PCR.
Various fluorescent transgenic lines have been generated that facilitate the imaging of hematopoietic cells (Bertrand and Traver, 2009).
Future studies will be needed to determine the exact cause of heterogeneous expression in various notochord transgenic lines.
Next, we transformed the Gal4 vectors into various rsGFP-GUS transgenic lines to determine qualitatively which lines (1X, 2X, 5X UAS) expressed more GFP and/or GUS.
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