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The differential abundance of various transcripts in any particular cell type is a well recognized obstacle for the efficient high-throughput analysis of cDNA libraries [ 18].
Examination of hTERT alternative splicing forms showed that there was no difference between the various transcripts in the treated compared to the nontreated cells.
Quantitative real-time PCR (qPCR) has been established as one of the standard methods for semi-quantitative measurements of the abundance of various transcripts in virtually any biological sample.
To gain a broad overview of the representation of various transcripts in different tissues, we compared the number of zebra finch homologues assigned to the different pools (o rgans, blood or feathers, Table 3, Fig. 7).
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In Arabidopsis, various transcripts involved in regulation of P metabolism have been identified.
Deconvolution techniques have been proposed to extrapolate from the Exon ST 1.0 data the various transcripts present in the sample, but the complexity of the task may be beyond current computational resources.
The differences in relative expression levels of the various transcripts will result in a large number of redundant transcripts within these libraries.
High throughput sequencing approaches has led to characterizing various transcripts of Vitis, in particular microRNAs, small RNA molecules that have been shown to play a central role in gene regulation within several important biological processes.
Using molecular biological methods, we have determined the relative abundance of various transcripts of genes during in vitro sepal ripening and in tomato fruit pericarp at three stages of development.
This analysis confirmed the enrichment of various transcripts (Klf4, Atf3, Nefh) in the GFP+ cells.
In vitro treatment of cells by cisplatin induces alterations of splicing in various transcripts [ 15, 24, 25].
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