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The true and false positive rates were determined at various stringency levels.
Using various stringency levels, the number of tissue-specific genes obtained for each stringency level was determined for fetal liver as well as placenta.
We demonstrated how various stringency criteria could be deployed to check data robustness.
Further, we have used various stringency criteria in order to check the method's robustness.
To determine if this possibility was real, BAC-FISH was done separately with ten randomly selected misincorporated BAC clones under various stringency conditions (77%, 87%, 93%and98%8%and98%
Using a False Discovery Rate with a significance level set at 0.05, we identified two subsets of genes/ESTs that were differentially expressed between males (n = 1803) and females (n = 1985) at various stringency levels (absolute fold change > 2.0 > 2.5, > 3.0, adjusted probability value <0.05, Figure 1B and Additional File 2 Table S2a and S2b).
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The trees were generated on full and reduced data sets, in which redundant sequences, divergent regions, and unique positions were removed at various stringencies (supplementary table S1, Supplementary Material online).
Poly P plays an important role in mediating bacterial survival under various stresses and stringencies [9], [12].
Utilising high stringency screening conditions, various peptide sequences containing multiple histidine, tryptophan, and/or tyrosine residues were identified amongst the different phage peptide sequences isolated.
It is therefore not surprising to find studies differing in their stringency for the various criteria for defining an EMT.
We have identified a striking variation in the number of assembled contigs with matches in the RefSeq database (Table 2) and suggest that this is likely to be the result of several different factors, including stringency of cutoffs in BLAST searches and variation in the representation of various tissue types in the RefSeq database itself.
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