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To do this, we considered the distribution of exons over various splice types in AS genes.
The roles of the various splice variants and the effects of single-nucleotide polymorphisms (SNPs) in cancers are discussed.
FGF signalling comprises a very complex network, including 24 FGF family members and four different receptors, whose signalling outcome depends on various splice isoforms and on the multifaceted crosstalk between different pathways58.
To test this hypothesis, the authors used commercial Math5 peptide antisera to probe extracts from cells transfected with various splice products (cf. Figure 1h).
The various splice forms of the PML I transcript were amplified by RT-PCR using the following primers: hPMLE3F 5'-CTTGCATCACCCAGGGGAAA3' (sense), and hPMLE9R 5'-CCGGTTCACCGCAGCCAGCT-3' (antisense).
We performed ZFN-mediated targeted mutagenesis of the C. griseus BAX gene to dissect the importance of various splice site sequences in their normal, endogenous chromosomal context.
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This allowed us to compare each pair of developmental stages for alterations in various splicing events.
According to our analyses, differently spliced exons seem to exhibit significantly different properties, which in part may give rise to various splicing frequencies.
Analysis of the nucleotide sequence showed that Xiap-ps1 gene is devoid of intronic sequences found in Xiap and analysis of the various splicing variants showed that Xiap-ps1 shows similarity to the regions in common to all variants, starting 7 nucleotides upstream of the initiation codon in exon 2 of Xiap (Fig. 1b).
Previous studies have demonstrated that the depletion of various splicing factors induces defects in mitosis 27.
The recognition of splice sites requires multiple RNA binding proteins to bind to various splicing signals in pre-mRNAs.
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