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A recurrent topic in phylogenomics is the combination of various sequence alignments to reconstruct a tree that describes the evolutionary relationships within a group of species.
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Among these, various sequence alignment studies have uncovered evolutionarily conserved protein domains in networked systems [ 32, 35– 35].
Furthermore, these probabilities can be estimated in a relatively efficient manner, making the pair-HMMs an attractive choice for various sequence alignment problems.
To design specific primers (Table 2), dissimilar regions were identified between target sequences using various sequence alignment tools available in the computer software BioEdit (Ibis Biosciences, Carlsbad, California, United States, 92008).
Although the alignment scores were very close to each other, given the consistency of the results obtained from the various sequence alignment tools used, the following observations can be made: (i) YNG1 shows the highest degree of sequence homology to ING1, (ii) YNG2 shows considerable homology to ING4 and ING5, and (iii) Pho23 and ING3 are similar to each other.
In order to detect the conserved domains and motifs among various SODs, multiple sequence alignments were carried out.
These motifs in combination with the overall catalytic domain sequence can be used to identify genes in genomes that encode protein kinases by various approaches such as sequence alignments and hidden Markov model searches.
Various sequence analyses and manipulations involving sequence alignments, conceptual translation, protein sequence examinations and calculations (e.g. molecular weight, isoelectric point) were carried out with Geneious v5.6 and v6.
Figure 5A and C shows the alignment sensitivity, and Figure 5B and D shows precision for various sequence identity bins in reference alignments.
Prediction of the 3R protein secondary structure was performed based on sequence alignments with various 3R homologs by using the JPred program.
The multiple sequence alignments of various cortactin proteins were constructed using Basic GeneBee ClustalW 1.83 [ 78].
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