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Among these, various sequence alignment studies have uncovered evolutionarily conserved protein domains in networked systems [ 32, 35– 35].
Furthermore, these probabilities can be estimated in a relatively efficient manner, making the pair-HMMs an attractive choice for various sequence alignment problems.
To design specific primers (Table 2), dissimilar regions were identified between target sequences using various sequence alignment tools available in the computer software BioEdit (Ibis Biosciences, Carlsbad, California, United States, 92008).
Although the alignment scores were very close to each other, given the consistency of the results obtained from the various sequence alignment tools used, the following observations can be made: (i) YNG1 shows the highest degree of sequence homology to ING1, (ii) YNG2 shows considerable homology to ING4 and ING5, and (iii) Pho23 and ING3 are similar to each other.
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A recurrent topic in phylogenomics is the combination of various sequence alignments to reconstruct a tree that describes the evolutionary relationships within a group of species.
The multiple sequence alignment for a given protein family is usually different in the various standard collections of protein families and the disagreement between protein datasets demonstrates that all multiple sequence alignment methods produce errors.
K-shortest path is used in various fields like sequence alignment problem in molecular bioinformatics, robot motion planning, path finding in gene network where speed to calculate paths plays a vital role.
Thus, we compared the H. pylori proteome to a set of known proteases from various organisms using sequence alignment techniques.
Various sequence analyses and manipulations involving sequence alignments, conceptual translation, protein sequence examinations and calculations (e.g. molecular weight, isoelectric point) were carried out with Geneious v5.6 and v6.
Figure 5A and C shows the alignment sensitivity, and Figure 5B and D shows precision for various sequence identity bins in reference alignments.
Sequence alignment has various applications like identifying homologous proteins, analyzing gene expressions and mapping variations between individuals.
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