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The scalability of this multiplex system, with its open-platform design and ability to use various sample types, makes the GTPlex applicable for use in multiple settings.
In this study, we applied different collection methods and a combination of direct amplification and lysis to various sample types in combination with the Qiagen Investigator 24plex GO! Kit.
Metabolites related to purine metabolism and protein degradation were correlated to neutrophilic inflammation in the various sample types and diseases, and several markers correlated with lung function [17].
Even in this basic initial form, it opens the possibility of searching for various sample types, organs, and ICD-10 disease groups (with a dozen filtering criteria) across European biobanks, and the result list can be narrowed by physical location.
Thus these have been utilized as screening methods for comparison of protein profiles from various sample types among populations of healthy and diseased patients for the pursuit of disease biomarker detection.
Where sequences from the various sample types clustered together, those sequences were hand aligned in Sequencher 4.5 and blasted in NCBI to determine putative identification.
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Moreover, single amplicons of expected sizes were obtained with both primer pairs from various environmental sample types, including aphid cadavers, plant material, and soil.
The sample key for the experiments used here is presented in Additional file 1; this key connects the individual experiments with the various plant sample types and experimental variables.
In previous epidemiological surveys and clinical studies, estimation of headaches in children and adolescents has been carried out by means of different assessment methods, the use of various informant sources and sample types.
Ten studies reported in 12 papers are characterized by various study designs and sample types.
These were included to examine the performance of the assay across a range of sample types, including various cooking methods (fried, baked, boiled, grilled, cooked in sauce) and serial DNA dilutions (50, 40, 30, 20, 10 ng/μl).
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