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Subsequently, the CMV promoter, chicken NUDT9 cDNA, mCherry cDNA, and a B-actin-Neomycin resistance cassette were inserted using various restriction sites.
Aliquots of genomic DNA isolated from T. brucei cultures were digested with various restriction enzymes.
On Fig. 4 we provide a physical map of Alu repeats cleavage by various restriction endonucleases.
Using various restriction enzymes and probes, Southern blotting has been effectively used to determine haptoglobin genotypes [ 16, 20].
Enzymes including T4 DNA ligase, calf intestine alkaline phosphatase, DreamTaq polymerase and various restriction enzymes were obtained from Fermentas.
Genomic DNA (3 5 μg) was digested with 30 units of various restriction endonucleases in a total volume of 30 μl at 37°C for two hours.
Similar(41)
Though some of Mill's pronouncements suggest that causing harm is a necessary condition of restricting liberty, closer inspection suggests that Mill countenances various restrictions on individual liberty that appear designed to benefit others, rather than prevent harm.
They continued to defend their various restrictions.
Last year, the site opened to the general public, but it still maintains various restrictions.
Various restrictions and requirements — like parking-space minimums and restrictions on lot size and building height — would disappear.
Most of the laws involving magic in his world seem to be various restrictions on its use.
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