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For the in vitro cytotoxicity experiment, 1 × 10 of luciferase-expressing MOVCAR (MOCAR-luc) cells were incubated with 0.5 μg/ml of one of the various proteins on 96-well plate for 6 hours and treated with 2 × 10 OVA-specific CTLs.
The yield of various proteins on the laboratory scale (1 2 l of shake flask culture) was in the range of tens of milligram.
To assess the relevance of the altered expression levels of various proteins on the clinical outcome in breast cancer patients, we performed survival analysis of up- and down-regulated proteins selected in Tables 1 and 2 using an online survival analysis tool.
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The various proteins of each HD occur on both sides of the basal cell plasmalemma and with anchoring filaments (AFs) form a bridge linking the interior of the basal cell to the exterior connective tissue and ultimately the distal phalanx.
After several washes with TBST 1 ×, the membranes were soaked in ECL solution (GE Healthcare) and the expression level of various proteins determined on the Amersham hyperfilm ECL (GE Healthcare).
After in vitro phosphorylation using [32γ-P] ATP, various proteins were resolved on one-dimensional 8% SDS-PAGE, stained with Coomassie Blue and autoradiographed.
Although a number of specific cysteine and methionine residues on various proteins have been identified as participants in redox signaling, their counterparts on many other proteins known to be vital to redox signaling remain unidentified.
Comparison of FAMCS with other methods on various proteins shows that FAMCS can address all four requirements and infer interesting biological discoveries.
The effects of 25-OCH3-PPD on various proteins and their interrelation are summarised in Figure 6.
MMPs exert proteolytic activities on various proteins including many ECM components, and are thus central to ECM formation [ 26].
We have also performed BLAST searches on various proteins found in the genome of C. jejuni RM1221 to identify proteins that are unique to the Campylobacter species.
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