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In phylogenetic trees based on 16S rRNA and various proteins sequences, the methanogenic archaea form at least two distinct clusters (see Fig. 1) [ 13, 29, 34, 56, 106].
The various Xin protein sequences were assembled in FASTA format in YooEdit V1.71 (http://www2s.biglobe.ne.jp/yex/YooEdit/).jp/yex/YooEdit/
To build datasets for phylogenetic analysis, various apicomplexan protein sequences were used as queries to search orthologs from the NBCI non-redundant protein databases.
The phylogenetic distances between various TMC protein sequences were calculated with ClustalW [ 11] and the resulting distance matrix Additional file: 1 (A) was visualized as a tree diagram with centered node position using the Phylodendron phylogenetic tree printer available at.
A Hidden Markov Model (HMM) of the human myotubularin multiple sequence alignment was constructed using the HMMER program package, which was then used to search the various eukaryotic protein sequence datafiles (program commands "hmmbuild", "hmmcalibrate" and "hmmsearch", threshold E = 1).
Alignment of various Leptomonas Rab protein sequences showing sequence similarities and domain conservation among them colored red based in their percentage similarity.
The multiple sequence alignment of the various rice MT protein sequences (based on in silico predictions) with the protein sequence of MT gene cloned in our laboratory from Indica rice genotype Pokkali, OsMT1e-P [Genbank: EU684548] has been shown in Additional file 1: Figure S1A.
The experiments presented in the current paper focus on various bidimensional HP lattice protein sequences.
Alignment of various Leishmania donovani Rab protein sequences showing sequence similarities and domain conservation among them colored red based in their percentage similarity.
HMM searches for PAS domain proteins in predicted Oikopleura protein database and BLAST searches using various bHLH-PAS domain protein sequences of vertebrates and invertebrates identified three bHLH-PAS domain proteins in Oikopleura.
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