Sentence examples for various protein isoforms from inspiring English sources
BPAG1-b is the major muscle-specific isoform encoded by the dystonin gene, which expresses various protein isoforms belonging to the plakin protein family with complex, tissue-specific expression profiles.
Consequently, the expression profile of the various protein isoforms is often different from normal tissues [6][4].
To detect the potential presence of various protein isoforms we prepared lysates of several cell types and did Western blot with an antibody specific for the carboxy terminus, which is common to all three isoforms.
In mammals 4 reticulon genes are known (RTN1 RTN4), which encode various protein isoforms [1,2].
In contrast, the western blot results demonstrated the presence of various protein isoforms at varying expression levels that diverge from the RT-PCR findings (Fig. 2B).
Alternative splicing plays a critical role in the regulation of eukaryotic gene expression, allowing a single gene to generate various protein isoforms that have different biological functions [ 1– 3].
As shown in Fig. 2, expression of various p63 protein isoforms was robustly detected in several of the cell lines tested.
ETV1 was hardly expressed in control benign prostate hyperplasia sample G277. Figure 4B schematically represents the predicted composition of the various ETV1 protein isoforms that will be produced.
We found early expression of various MyHC protein isoforms, in a population of proliferating mononucleated myoblasts with an elongated spindle-shaped morphology without fusion.
Western immunoblotting with monoclonal antibody to the v6 splice variant revealed expression of various CD44v6 protein isoforms within the range of 97 220 kDa in control cells, similarly to the lanes obtained with the control peptide.
Sequential activation of the phospholipases generates the second messengers inositol 1, 4, 5-trisphosphate (IP3), diacylglycerol (DAG), and arachidonic acid (AA), which are required for Ca2+ mobilization, the activation of various protein kinase C isoforms (PKCs), and the production of prostaglandin (PG) and other metabolites of AA, respectively.
