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Various nucleotide changes and indels were identified within the population of 31 accessions at each locus in the 5′ UTR and coding region (Table 2).
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Transformed plasmids with the desired nucleotide changes were confirmed by DNA sequencing.
Nucleotide variability was investigated and a total of 139 nucleotide changes were found in the LacS gene while 40 nucleotide changes were found in the sequences of the LacZ gene.
All nucleotide changes were located on the bacterial chromosome, whereas no polymorphisms were detected in the plasmid sequence.
Our analysis also suggests substantial variation in mutation rates even among mutations involving the same nucleotide changes.
Different letters indicate significant differences among various nucleotide substrates (Duncan's multiple range test, P < 0.05).
(A) MamK polymerization in the presence of various nucleotide states was assessed by a pelleting assay.
Recently, a novel strategy was developed to generate targeted precise nucleotide changes without introducing DSBs.
That connection was not elucidated to the level of specific nucleotide changes in DNA until recently.
Open image in new window Figure 2 Catalytic efficiencies and reaction processes of MamK towards various nucleotide states.
The technical sensitivity of this test is estimated to be >99% for single nucleotide changes and small insertions and deletions.
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