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Various negative controls were processed in parallel (a minimum of 18 and 4 non-human specimens and 16 and 3 extraction blanks in the Linköping and Stockholm laboratories respectively).
Various negative controls were included in the PCR reaction to ensure specific amplification.
To prevent contamination, all working steps were carried out in dedicated ancient DNA and/or special high-throughput sequencing laboratories, and various negative controls were applied.
Our array covering 12,000 cDNA probes for 10,000 non-redundant genes and various negative controls as well as nylon arraying of PCR-amplified probes and hybridisation of [αP]dCTP-labelled mRNAs have all been extensively described and validated in a previous report [ 21].
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It is important to calculate the actual overlap rate, and various negative and positive control overlap rates, and calculate a resulting P value for the differences.
EFs were not significantly different between hydroponic solution and various tissues from the negative controls, with a range from 0.484 ± 0.015 to 0.493 ± 0.007 for 5-OH-PCB91 and from 0.496 ± 0.006 to 0.521 ± 0.004 for 5-OH-PCB95 in dead poplars during the 10 day exposure.
The GeneChip was originally designed to represent 82,000 unique maize expressed sequence tag (EST) clusters and genes as well as various negative, spike, and transgenic control genes [ 28].
Again, the observed differences in invasiveness were not due to a significant difference in the cell growth among the negative controls and various treatment groups.
Positive controls containing tubes of HPV 16 DNA diluted at various strengths (0.01 10 pg/tube) and negative controls containing the PCR master mix but no DNA template were included in all assays.
The remaining 289 features contained various positive and negative control probes.
The results were represented as the logarithmic ratio of gene expression between the viral infected samples and their corresponding negative controls at the various time points.
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