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MUPAC could efficiently introduce various mutations at multiple specific sites within a gene.
In addition, various mutations at Lys revealed that the channel does not well tolerate alanine, isoleucine or arginine at this position, which might be an additional indication that Lys indeed plays a structural role in gating.
Among the 217 porB1B+ isolates in this study, only five had no mutations at the 120 and 121 sites, while the other 212 had various mutations at these sites, in which 76.9% (163/212) had double-site mutations (G120K/A120D, G120K/A121G and G120N/A121D in 135, 19 and 9 isolates).
In the 217 porB1B+ gonococcal isolates, only 2.3% (5/217, three belonging to the IB3/6 serotype and two belonging to the IB3 serotype) had no mutation at the 120 and 121 sites (G120 and A121), while 97.7% (212/217) showed various mutations at the sites.
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Further PCR-based genotype analysis demonstrated various mutations occurred at the SlitBLOS2 specific target site.
On the basis of the stepwise cleavage model initially proposed by Ihara and colleagues along with the data generated in this work and others, we can propose models depicting how GSMs, iGSMs, and various mutations that alter charge at the interface of the APP ectodomain and TMD modify γ-secretase processivity.
A wide range of phenotypes have been reported in association with various mutations in BMP4, ranging from anomalies evident at birth to adult onset colorectal cancer.
Results: Various mutations were detected including C to T point mutation at nt 1653, A to T and G to A contiguous point mutations at nt 1762 and 1764 in the core promoter region, and G to A point mutation at nt 1896 in the precore region, but no common mutations were detected that were directly related to the virus titer from earlier reported mutations.
Various mutations were identified, primarily in Vβ residues that are located at the TCR SEC3 interface.
Headed by Pedro "Busy P Winters, Ed Bangerr captured a crossover moment between a generation of young clubbers who were picking at the leftovers of electroclash, and enamoured with the various mutations of hip hop, electro, techno and house that were being called "wonky", "glitchy"; hackneyed terms, sure, but these sounds were searching for a playfulness that Ed Banger embraced in full. .
De Silva explained how brain cancer cells and their various mutations make ideal subjects for testing.
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