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Numerous primers were constructed incorporating various mismatches with a target sequence on the BKV T antigen gene.
The various mismatches between circulating and vaccine strains may have led to lower than expected estimates of VE.
However, more recently, the issue of specificity has been questioned as several groups have demonstrated that various mismatches between the guide strand of the siRNA and the mRNA target do not abolish the silencing effect of the siRNA [37], [38].
Therefore, an important next step is to characterize the composition of the mutagenic and non-mutagenic repair intermediates that assemble on the various mismatches.
In principle, even between incompatible crosses, certain metabolites, particularly those that require only trace quantity to produce dramatic effects like signal molecules, phytohormones and siRNA species etc., may be released from the donor pollens and enter the recipient stigma cells, thus may conceivably produce various mismatches and elicit a stress response.
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Especially, the effect of various mismatching conditions for RAC performance has been given.
The asymmetric primer design and use of Taq MutS were reported as innovative background suppression technologies employed by SMAP 2, but Taq MutS is known to display differential affinities for various mismatch combinations, and hence may not be entirely effective for all possible applications.
We evaluated the effect of parameter choice on ortholog recovery by computing human-zebrafish gene order alignments using various (mismatch × gap) -penalty combination and apart from that default parameters.
This assay was also able to discriminate the perfect complementary DNA target against various mismatch targets with a discrimination ratio of 3.8, 5.7 and 6.6 fold for the single-base, three-base mismatch and non-complementary oligonucleotides, respectively.
Recently, BRCA1 has been found to reside in a large DNA repair complex that includes various mismatch repair proteins including hMLH1 and hMSH2; thus, it plays a role in DNA repair [ 208].
We examined proofreading of Klenow fragment with DNAs containing various base mismatches.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com