Sentence examples for various isolates were from inspiring English sources

Trophozoites of the various isolates were cultured axenically in TYI-S-33 medium supplemented with 10% adult bovine serum [ 48].

Nucleotide sequences obtained from various isolates were assembled using the software programs BioNumerics (version 5.10) and BioEdit (version 7.0.9.0), and aligned with GenBank reference sequences using the Clustal X application within BioEdit.

Nucleotide sequences obtained from various isolates were aligned with GenBank reference sequences using the Clustal X application within BioEdit software (version 7.0.5.3), and compared with published sequences in the Lahey organization (http://www.lahey.org/studies).org/studies

The various isolates were inoculated on brain heart infusion agar that was supplemented with 10% horse blood, amphotericin B 20 μg/mL (Fungizone, Bristol-Myers Squibb, Epernon, France), and Vitox (Oxoid) (blood agar).

In similar studies conducted in Nepal, significant growths of various isolates were also detected by Shah et al. (3.7 %) in 2001 [ 25] and Ansari et al. (4.4 %) in 2011 [ 26].

The antimicrobial susceptibilities of the various isolates are detailed in the Table 1.

However, this evolution is so rapid for some genetic markers that complete knowledge of the relationships of various isolates is not clear, especially for isolates collected from outbreak cases, so that fine-scale epidemiological studies are not possible in all instances.

> -wrap-foot> Three colonies obtained from each of the various Nocardia isolates were analyzed.

DOI: http://dx.doi.org/10.7554/eLife.01322.003 A summary of mutation types detected in the various sequenced isolates is presented in Table 1.

The entire complement of mutations found in all isolates from the four evolved populations, both those sequenced for this study and those analyzed in the earlier study (Harris et al., 2009), are provided in Supplementary file 1A.> -wrap-foot> The prominent genetic alterations present in the various evolved isolates are not identical.

Various organisms isolated were Staphylococcus aureus, vancomycin resistant enterococci (VRE), Escherichia coli, Pseudomonas aeruginosa, Pseudomonas stutzeri and Klebsiella pneumoniae.