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Skp1 and its various isoforms were purified essentially to homogeneity and extensively dialyzed simultaneously against CD buffer [20 mM sodium phosphate (pH 7.4), 50 mM NaCl, 1 mM MgCl2, 0.1 mM EDTA, and 1 mM TCEP].
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While various isoforms are expressed throughout the body, the kidney and liver have been observed to express the highest levels of ALDH.
Expression of the various isoforms is tightly regulated by cell type and state of activation.
Multiple, alternatively spliced isoforms of Abi1 are expressed in mammalian cells, but the functional significance of the various isoforms is unknown.
The sequence similarity among the various isoforms is about 50 80%; the transmembranal helices are particularly well preserved [ 21].
Various phospholipase enzymes are involved in degradation of PtdCho to GPC, PCho, and Cho but the roles of the various isoforms are still not fully elucidated.
However, the serotype-specific protection conferred upon the fish by agglutination/immobilization antigens as shown by Hatanaka (2008) [ 63] and the existence of various isoforms are some obstacles that need to be overcome before this protein can be developed and used as a vaccine against C. irritans.
After the various NOS isoforms were identified, inhibitor development proceeded rapidly.
Although both DN calpain-I and calpain inhibitor-I suppressed calpain activity in MVECs as measured with a calpain fluorescent substrate assay, our experiments do not identify which of the various calpain isoforms were inhibited.
We followed the steps usually implemented for differential gene expression analysis, so various gene isoforms were not of interest.
Various human PTPN22 isoforms were identified from the GenBank database or amplified directly from human T cells.
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