Historically, overexpression of [NiFe]-hydrogenases has been complicated by the lack of co-ordinated expression of various biosynthetic genes required for cofactor insertion and other post-translational modifications.
NRF2 is a nuclear-encoded transcription factor that binds and activates various mitochondrial genes required for electron transport and oxidative phosphorylation [ 6].
To dissect the molecular mechanisms that underlie the development and physiological function of glial cells, glia-targeted manipulation of various genes is required.
On the other hand, the existence of many more mutants that seem to be intact in these surface structures but exhibit reduced biofilm phenotype showed that indeed various genes are required for the proper development of biofilm.
We unambiguously identified three radical SAM methyltransferase-encoding genes required for various methylations at unactivated carbons yielding tert-butyl valine, methyl-proline, and β-methyl-phenylalanine residues, plus a gene involved in aspartate methyl-ester formation.
Using mCD8 GFP as a reporter to visualize the morphology of the MBs, we have been screening for genes required for various aspects of MB development through loss-of-function genetic mosaic analysis [25], [30] [34].
We identified 25 genes required for various aspects of planarian regeneration, five of which have also been functionally characterized by other labs [ 21, 27– 30].
The pheromone response, which is shared by the two cell types, activates genes required at various steps throughout the differentiation process, with entry into meiosis being the most downstream event reported to rely on the signal [ 7].
Identifying more genes required for the various aspects of brain development is essential for elucidating further how the complex brain develops.
This is suggested to increase the availability of RNAP for activation of genes required for survival under various stressful conditions [ 7].
