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This work establishes a system for tight, tunable control of mammalian gene expression that can be used to explore the functional role of various genes as well as to determine whether a phenotype is the result of a threshold response to changes in gene expression.
Q-PCR was performed for various genes as previously described [5] and results normalized to β−actin levels.
2 μg of total RNA was reverse transcribed into first strand cDNA and subjected to PCR amplification for various genes as mentioned in Table 2 [ 23].
The second protein, LuxR, binds to the AI molecule to form a complex, which subsequently activates transcription of various genes, as shown in Figure 1 (a detailed description of this molecular pathway is provided by Garcia-Ojalvo et al. [ 1]).
Many lines of evidence suggest that poly(ADP-ribose) polymerase-1 (Parp-1) is involved in transcriptional regulation of various genes as a coactivator or a corepressor by modulating chromatin structure.
DHPLC has been commonly used for mutation screening of various genes as it is rapid, cost-effective and highly sensitive detecting 93 to 100% of mutations that were observed by DNA sequencing [ 47].
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On the other hand, PGC-1β was shown to regulate the expression of various OXPHOS genes as well as NRF1 and ERRA [ 27].
Polymerase chain reaction (PCR) primers to amplify various zein genes as well as ZmO2 and ZmPbf are described elsewhere (Song and Messing 2003; Wu and Messing 2009; Miclaus et al. 2011).
Resequencing of the various MODY genes as well as the genes responsible for other monogenic forms of diabetes (dominant and recessive, syndromic and nonsyndromic) is a goal of the National Institutes of Health funded T2-GENES Consortium, and these studies will help elucidate the role of monogenic diabetes genes in the etiology of type 2 diabetes.
SIRT1 acts as a nutrient sensor involved in the regulation of various gene expressions as well as modulation of important signal transductions either directly or indirectly through its unique epigenetic effects, which ultimately influence the regulation of longevity [14].
They include various genes such as those for signal transduction, transcription, carbohydrate metabolism as well as those of unknown function.
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