Various gene expression analysis approaches, including microarrays, have identified aberrantly expressed miRNAs in OC [ 9, 11- 26], of which some are related to progression [ 13], outcome [ 18- 24] and chemotherapy resistance [ 23- 25].
We first quantified by quantitative PCR (qPCR) the relative expression levels of significantly differentially expressed genes from the MAXRS method, exhibiting various gene expression levels and fold changes (see Figure 3; the fold change of the selected genes varied from 1.6 to 8.4).
In the future, we will assess samples from total body irradiation patients to validate various gene expression signatures and assay chemistries.
MCF-7 cells were stably transfected with various gene expression plasmids as indicated and the transfected cells were selected in cultures for approximately 70 days.
Various gene expression signatures have been correlated to survival of NSCLC patients [17] [19].
Notably, such combinatorial analyses revealed a striking increase in the level of aminoacyl tRNA synthetases (aaRS) that could underlie the increase in protein biosynthesis predicted in prostate cancer by various gene expression studies.
