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Real time qRT-PCR validation was conducted using 10 target genes representing various fold change values, and results were normalized to 4 separate housekeeping genes.
The various fold change indicates different degrees of up- or downregulation between mRNAs and lncRNAs.
These differences in expression may have led to the various fold change values observed between these related miRNA members.
We used various fold changes in order to observe the differences in the number of candidates at each threshold.
Different proportions of genes (10%and30%0%) were randomly selected as differentially expressed genes with various fold changes (1.5, 2, 3, and 5).
For a given comparison, one cell line was selected as the baseline reference, and transcripts that exhibited various fold change (ANOVA, P < 0.05; Benjamini and Hochberg correction for false discovery rate) relative to the baseline were considered differentially expressed.
Similar(51)
"Each ballot opens up and we slice it apart at various folds, making little ballots out of each master," explains Frank Johnson, who was one of Pricewaterhouse's chief counters from 1976 to 1997.
The features of these interactions in different structural classes of globular and membrane proteins, and in various folds have been delineated.
Getting the details right "required many hours of staring," Nicholls says, as he and Vinther studied how to untangle the overlapping pigmentation patterns left in the various folds of the flattened fossil.
Thus, we compared the sensitivity and specificity of beads array (Figure 6; left panel) and LNA array (right panel), using the TLDA results as a reference, under different normalization methods with various fold-change cutoffs (x-axis).
Proteins with various folds were selected: α helices, only β-strands and both α and β.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com