We set out to systematically develop and characterize monoclonal antibodies directed against various epitopes of ADM and investigate their potential utility as drugs to reduce sepsis-associated mortality in a mouse CLP model.
Struck et al.[5] describe the development of monoclonal antibodies directed against various epitopes of ADM and their ability to attenuate sepsis-induced mortality in severe murine sepsis (100% control group mortality within 2 to 3 days).
In the patients with PTU-induced AAV, no significant association was found between levels of antibodies against various epitopes of MPO and clinical parameters, including serum creatinine, ESR, C-reactive protein, numbers with organ involvement and BVAS.
Our recombinant system inherently supports the expression and delivery of various epitopes [ 8, 9, 13] and could be readily adapted if important, new peptides were identified.
While insertions of His-tag or M2e (7-23 aa) into the predicted external loop structures did abolish VLP formation, high yields of VLPs were obtained with all fusions of His-tag or various epitopes (13 - 27aa) from IAV and FMDV at the N- or C-terminal ends of CCMV CP or N∆24-CP.
We tested lines expressing EGFR with three different monoclonal antibodies (MAb) directed against various epitopes in the ectodomain of the EGFR (528, H-11, and 199.12) and QD-coupled secondary antibodies.
Normal and vCJD BHs were digested, PrPSc was centrifuged (Fig. 3C, lanes 1 6, input), and PrPSc pellets were treated with or without denaturant, and immunoprecipitated with antibodies recognizing the various epitopes of interest.
Tests involving epitope mapping were performed to probe the relationships of the various epitopes to that defined by the NCRC-11 antibody, and, of note, the three antigen preparations from different tumour sources were remarkably similar with respect to their relative levels of epitope expression and to their topographical distribution of epitopes.
We computed and compared the physico-chemical properties of various epitopes to understand their correlation with antibody-class specificity.
Epitope mapping was done by Dr Gergely Lukacs: Mr Pink recognizes various epitopes in NBD1, 7D12 sees epitope 531 540, 3G11 sees epitope 396 405, and 10B6.2 recognizes epitope 399 408).
