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The key message is that quality assurance and quality control are essential throughout the entire RT-qPCR workflow; from living cells, over extraction of nucleic acids, storage, various enzymatic steps such as DNase treatment, reverse transcription and PCR amplification, to data-analysis and finally reporting.
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The de novo biosynthesis of arginine in microorganisms and plants is accomplished via several enzymatic steps.
While much progress has been made in elucidating the mechanistic basis for various steps in protein trafficking, many aspects about the core components and enzymatic steps remain unresolved.
In conjunction, it is possible to deduce a functional conservation pattern for Gammaproteobacteria, where some metabolic maps contain a biosynthesis-related core of similar enzymatic steps, and some variable steps that include the degradation of various compounds.
They cannot reveal, however, the details of the enzymatic steps involved.
All enzymatic steps depicted here are inferred to occur within the apicomplexan plastid58.
Thus, in the studied contexts, flux control in glycolysis is concentrated in four key enzymatic steps.
Also, levels of oxidative stress reflected by various enzymatic and non-enzymatic antioxidants were measured.
We identified putative functional homologues of genes encoding 18 enzymatic steps of cellulose and heteroxylan biosynthesis (Supplementary Information section 5).
The steroid pathway in MA-10 cells has only two specific enzymatic steps.
Central carbon metabolism uses a complex series of enzymatic steps to convert sugars into metabolic precursors.
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