Sentence examples for various destination vectors from inspiring English sources
A major advantage of the various Destination vectors is that a combination of cDNAs/shRNAs/miRNAs can be expressed in the same cell, in either a constitutive or inducible manner.
The compatibilities of each Entry vector for various Destination vectors are summarized in Table 1 as well as represented schematically in Figures 4A for lentiviruses, and Figure 4B for retroviruses.
Primers were designed using the nearest neighbour algorithm [ 38] and open reading frames (ORFs) were PCR amplified from first strand cDNA with 5' attB1 and 3' attB2 linkers and then recombined with pDONR221 (Invitrogen) to give a set of entry clones which were sequence confirmed and then recombined with various destination vectors to give the expression constructs.
Figures S1 and S2 show various Destination vector backbones we tested for the FLAG (Figure S2B), GST (Figure S2C), Ubc9 (Figure S1E) and nuclear localization fusions (Figures S2A, S2C).
Entry vectors were set up in LR reactions to recombine the gene of interest into several destination vectors (expression vectors).
Several destination vectors were created for transient expression in N. benthamiana and stable rice transformation.
Entry vectors were set up in LR reactions to recombine the gene of interest into several destination vectors.
The ORFs within sequence confirmed attL containing entry plasmids were then recombined the various attR destination vectors described above to generate sets of expression plasmids.
The development of highly efficient, in vitro recombination cloning technologies (e.g., Gateway™ technology) recently made it possible to capture cDNA libraries in entry vectors that allow easy shuttling to various downstream destination vectors [ 15- 18].
The various combinations of Entry to Destination vectors tested are indicated in Table 3.
Based on the backbone of vector pCAMBIA (CAMBIA, Canberra, ACT, Australia), we designed and constructed a set of three T-DNA destination vectors for various tasks, carrying no plant selectable marker gene or genes encoding resistance to hygromycin (hptII, hygromycin phosphotransferase) and phosphinothricin (bar, phosphoinothricin acetyltransferase).
