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A color-coded scheme summarizing the expression data obtained in vivo with the various constructs is shown in Figure 4A. Figure 4B shows some representative examples of GFP fluorescence driven by the various constructs of the prep1.1 promoter injected in zebrafish embryos.
Transient expression analysis using various constructs of the F3H promoter revealed that the TORE1 insertion is indeed involved in reduced promoter activity.
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We used isothermal titration calorimetry (ITC) to study the interaction between IP6 and various constructs of Btk.
To further identify the protein domains involved in this interaction, we generated various constructs of Tau and BIN1 (Fig. 1a).
(B) Methyltransferase activities for various constructs of METTL3-METTL14.
Endothelial cells transiently transfected with various constructs of PCMT, i.e. overexpressing wild type PCMT or negative dominants, were used to investigate the role of protein methylation during apoptosis induced by oxidative stress (H2O2; 0.1 0.5 mM range).
The factor loading of the various sub constructs of the Entrepreneurial orientation construct i.e. innovation, proactiveness and risk taking had a value higher than 0.694.
The various deletion constructs of the putative promoter were transfected into U937 cells along with pSVβGal vector in a ratio of 8∶2.
After receiving additional questions regarding the various psychosocial constructs of the selected lifestyle behaviour, they receive personal advice on this behaviour which aims at helping to change this health behaviour during the first year of the study.
Briefly, the full-length and various deletion constructs of the OsSec18 and Os60sP0 genes were inserted into pGBKT7 and pGADT7 (Clontech, Mountain View, CA, USA) according to the manufacturer's instructions, and the constructed plasmids were transformed into the yeast strain AH109 using the LiAc method [ 28].
In addition we provide a restriction map of the murine wt1 locus which should prove useful for the cloning of various constructs designed for the generation of mouse models.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com