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We first analyzed the expression and activity of various complexes of the mitochondrial electron transport system by Western blotting using a cocktail of monoclonal antibodies optimized for the detection of one subunit of each of the five OXPHOS complexes (20 kDa subunit in Complex I, SDHB in Complex II, core 2 in Complex III, COX II in Complex IV and F1α in ATP synthase).
Various complexes of cyts c with other protein partners have also been prepared to study interprotein ET pathways.
In the last decade it has become clear that various complexes of the mitochondrial ETC have a multifaceted role in the execution of cell death.
We present here crystallographic structures of various complexes of PKAc that provide new information about the detailed chemistry of the catalytic mechanism.
Conformational and dynamics data for a wide range of time scales have been collected for side chain residues of various complexes of human DHFR that represent intermediates in the catalytic cycle.
This is largely due to experimental methodology applied to complex enzyme kinetics and substrate utilization by various complexes of the electron transport chain (Lesnefsky and Hoppel, 2006; Tatarkova et al., 2011).
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By combining the predicted growth rate of individual cavities with the emerging cavity population density determined experimentally, a quantitative understanding of the various complex dependencies of cavitation has been obtained.
The relative ratio of the various complexes will be a function of numerous parameters (e.g., pH, ionic strength, etc).
The optimizing method of this paper can be used to optimize various complex problems of engineering applications.
Conservation of epitopes and receptors, however, introduces additional constraints on the concentrations of various complexes.
Actin always exists as a part of various complexes.
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