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It should be noted that the first alternative exon of HR-HPVs' early transcripts in E6 ORF region can be alternatively spliced and lead to at least four species of mRNA transcripts with various coding potential in HPV16 (5′ splice site at 226 nt and three alternative 3′ splice site at 409 nt, 526 nt, or 742 nt) (Zheng and Baker, 2006).
We checked other gene annotations from UCSC "KnownGene", "RefGene", "mRNA", "EST", and "EnsemblGene", and found various coding signals outside the scope of Entrez gene annotations (Table S12).
In addition, there were no statistical differences between gallstone carriers and controls for the various coding region variants: rs41281680/p.A43, rs55971546/A98I rs41281678/L169I, rs188096/A171S9, rs188096/A171S.
Database search revealed that ~80% of the somatic and ~60% of spermatozoal transcripts have significant homologies (>85%) with various coding genes across the species.
Also, several studies have reported that the sensitivity and specificity of various coding abstraction methods perform poorly compared to structured chart review [ 19– 219.
To score written reflections various coding schemes have been proposed, using from three to seven categories [ 32, 33] and introducing a variety of indicators [ 34].
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