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Confocal laser microscopy was used for looking at the expression of various cell specific markers tagged with Green Fluorescent Protein (GFP).
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Specifically, we have shown that various cell-specific members of the Fkh TF family associate with the same binding sites within a single enhancer, thereby regulating the different spatiotemporal expression patterns of the associated target gene.
We monitored cellular growth, the emergence of Sox1-GFP+ NPs and the appearance of various cell-specific markers in these cultures.
In this organism, various cell-specific promoters can be utilized for cell-specific expression to determine the function of a gene in a specific cell.
Retinal sections were stained with DAPI and various cell-specific markers, e.g. Prox1, Chx10, and Calretinin for retinal cells in the INL.
Using mouse NS cells constitutively expressing GFP, we confirmed the differentiation potential toward neuronal and glial fates by monitoring the appearance of various cell-specific markers.
To identify the neurons in which EAT-4 functions for thermotaxis, we introduced eat-4 cDNA under the control of various cell-specific promoters into eat-4 ky5) eat-4 ky5
Binding of the ligand to the P2X7 receptor can activate various cell-specific signaling cascades, including the IL-1β [ 19], TNFα – TRAIL [ 20], and the p38, JNK/SAPK [ 21] and NF-κB cascades [ 22].
Numerous delivery strategies have been proposed for different target cell types via various cell surface specific ligands [1].
For the various cell type specific markers four different culture wells per marker were examined for each of the three different groups, leading to the examination of 48 cell culture wells.
As a parallel approach by characterising the changes in the proteome of differentiating CSM14.1 cells we used various cell type specific antibodies to document the morphological and phenotypical alterations over time.
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