From our observations in various yeast cell samples, it is sufficient to use the value of α as 3. To determine the FDA staining principle which can be applied to yeast counting, we applied FDA/PI mixture to overnight cultured yeast cells.
The lowest-level SAGE library contains the sample information and gene expression data for various cell and tissue samples.
To determine peripheral blood hematological recovery and to quantify various cell subsets, blood samples were obtained using heparinized capillary tubes at the indicated time points.
For this purpose, we undertook a range of experiments applying our modified IN Cell Analyzer 1000 instrument to various dye and cell samples.
Our finding is consistent with other reports on elevated levels of MPs of various cell types in blood samples of TBI patients and animals subjected to TBI (Table 1).
Total RNA was extracted with Trizol (Shanghai Bio-Engineering Company) from various cell lines and tissue samples.
Reasons for the inconsistent detection of telomerase expression could be manifold, including different laboratory methods used for analysis and various cell contents in tissue samples.
There were significant correlations between both assay platforms when measuring total GR (exon 5/6) transcripts in various cell lines and patient samples, but not for a probe set that detects a specific, low abundance GR transcript (exon 1A3).
To investigate systemically if methylation of miR CpG islands represses transcription of miR genes, various cell lines and tissue samples with different methylation status of representative miR CpG islands must be used.
