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Taken together, Nck adapters are not only employed in various cell conditions but also by different cellular compartments to generate or modulate specific cellular responses.
As a first set of validation categories, we utilized the yeast genome-wide datasets on in vitro TF DNA interactions available for the three TFs (Rap1, Mig1 and Abf1) from Mukherjee et al. (2004) and the in vivo ChIP chip data from Harbison et al. (2004) for more than 200 TFs in various cell conditions.
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By regulating the flow rate and nutrients of culture media, which are essential for relevant in vivo-like modeling, various cell culture conditions were also obtained (data not shown).
To determine whether the various cell culture conditions have an effect on the pluripotency of maGSCs, we compared the tumor growth of maGSC Stra8 SScellslls cultured under the conditions I to IV after subcutaneous injection into SCID/beige mice.
Thus, we developed an in vitro cultivation system by purifying BCR/ABLp210+ LT-HSCs and BCR/ABLp185+ pro-B cells from moribund mice and maintaining them under various cell culture conditions.
In situ confocal micro-Raman spectroscopy was used to probe the water distribution in an electrolyte membrane in a polymer electrolyte fuel cell (PEFC) under various cell-operating conditions.
When possible, we included gene sets curated from connective tissue such as fibroblasts, and we also included the Gaffen gene set (28), which more generally includes genes from various cells and conditions.
However, well-described models applying this methodological approach with different cell lines and various cell plasticity modulating conditions are missing.
Moreover, despite the possibility of examining multiple TFs in a single experiment [ 3, 4], it is still prohibitively expensive to test thousands of TFs in various cell types and conditions.
In the future, we plan to use the developed AFM platform to investigate the forces between nanoparticles and various cell lines under conditions where certain nanoparticle internalization mechanisms have been blocked.
Using a novel monoclonal antibody for Jmjd6, we investigated its nuclear localization and distribution under various cell culture and growth conditions in different human cell lines and primary mouse embryonic fibroblasts (MEFs).
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